Condition of membranes of erythrocytes of peripheral blood of elderly people with chronic tiredness and low level of tolerance to physical load

Keywords: chronic fatigue syndrome, exercise tolerance, peripheral blood erythrocytes, osmotic resistance of erythrocytes

Abstract

Purpose:  The aim of the work is to study the osmotic stability and morpho-functional features of peripheral blood erythrocytes of patients with chronic fatigue syndrome depending on the level of exercise tolerance and associated risk factors. groups of factors that may be the cause, so the development of measures to eliminate them is an urgent problem today, which requires the development of effective ways to correct chronic fatigue syndrome. It is known that the peripheral part of erythron actively responds to changes that occur in the whole body after the action of various factors, including those factors that lead to chronic fatigue syndrome.

Material and methods of research. The examination was performed on the basis of the Department of Vascular Neurology of the Central Clinical Hospital of Ivano-Frankivsk. The study involved 30 patients aged 55-65 years (mean age 60.6 + 1.2 years), who were divided into 3 groups: 1 gr. included 10 patients with chronic fatigue syndrome and a high level of exercise tolerance. The 2nd group included 10 patients with chronic fatigue syndrome and the average level of exercise tolerance, the composition of 3 gr. included 10 patients with chronic fatigue syndrome associated cardiovascular pathology (angina pectoris, hypertension) and low exercise tolerance (3 gr.).

Results. It is established that chronic fatigue syndrome proceeds in 3 phases and has natural stages of development which are characterized by three groups of etiological factors and the corresponding levels of teletransitivity to physical activity, each of which corresponds to a certain erythrocyte profile and level of osmotic stability of erythrocytes which are offered to use as prognostic and diagnostic characteristics. chronic fatigue syndrome.

Conclusions. The study of the quantitative composition of  peripheral blood erythrocytes and hemoglobin, as well as their ratio (color index) in patients with chronic fatigue syndrome on the background of low levels of exercise tolerance revealed a decrease in erythrocytes and hemoglobin by 42% and 25%, respectively, indicating the presence of anemic hypoxia. 2. In conditions of chronic fatigue there is a decrease in osmotic resistance of erythrocytes, as evidenced by a decrease in the number of osmotically stable erythrocytes with a gradual decrease in the concentration of NaCl solution (3.0%; 0.5%; 0.46%; 0.3%). 3. In chronic fatigue there is a deterioration of membranes in peripheral blood erythrocytes, as evidenced by a decrease in their osmotic stability and an increase in the number of irreversibly altered forms of РВЕ, as well as a decrease in peripheral blood erythrocytes, which is closely correlated with decreased tolerance to exercise.

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Author Biographies

Sergii Popel, Vasyl Stefanyk Precarpathian National University. Ivano-Frankivsk, Ukraine

popelsergij@gmail.com

https://orcid.org/0000-0001-9019-3966

prof. department physical culture and sport Vasil Stefanyk Precarpatian University,

Shevchenko str., 57, 76025, Ivano-Frankivsk, Ukraine

Ivan Меlnic, Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine

imelnik_64@ukr.net

https://orcid.org/0000-0002-2585-6134

Candidate of Medical Sciences,

Associate Professor of the Department of Dental Surgery,

Ivano-Frankivsk National Medical University,

Ivano-Frankivsk Hetman Mazepa Street 11 \ 14, Ukraine

Іhor Churpiy, Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine

ch.igor.if@gmail.com

https://orcid.org/0000-0003-1735-9418

professor, Ivano-Frankivsk National Medical University, 

Halytska str. 2, 76000, Department of Physical Rehabilitation,

Ergotherapy with a course of physical education, Ivano-Frankivsk City, Ukraine

 

Przemek Bejga, University of Zielona Góra, Polska

bejbej@o2.pl

https://orcid.org/0000-0003-4871-4689

professor, head of the research project

“Health promotion in pharmacies according to the patients +60”,

geriatric rehabilitation center, Elena Gurа, Poland;

  1. Szprotawska 21 Kożuchów 67-120, Zielona Góra, Polska
Zbigniew Śliwiński, Wroclaw medical University, Wrocław, Polska

popelsergij@gmail.com

https://orcid.org/0000-0003-4871-4689

professor,

Wroclaw medical University,

Wybrzeże Ludwika Pasteura 1, 50-367 Wrocław, Polska

References

Vdovina L.N. Razvitiye sindroma khronicheskoy ustalosti u sovremennoy molodozhi [Development of chronic fatigue syndrome in modern youth.]. In the collection: Social competence of the individual. collection of scientific articles. Yaroslavl, 2020. С. 37-41. .[in Russian]

Vnukov, V.V., Ovsyannikov M.V., Maslovskiy S.L. Rol' svobodnoradikal'nogo okisleniya v molekulyarnykh mekhanizmakh patogeneza [The role of free radical oxidation in the molecular mechanisms of pathogenesis]. Advances in modern natural science. 2014;2:33-34. https://doi.org/10.18097/pbmc20166202193 https://search.crossref.org/?from_ui=yes&q=erytrocytes. (date of application 11..06.2021) [in Ukranian]

Kozinets G.I. Shishkanova Z.G., Novozhderkina Yu.K.. Кonfiguratsiya i poverkhnost' kletok krovi v norme i patologii [Configuration and surface of blood cells in health and disease]. Moskva: Triada-farm, 2014; 5: 174.

Potapenko, A. Ya. Kyagova A.A., Tikhomirov A.M. Osmoticheskaya ustoychivost' eritrotsitov [Osmotic stability of erythrocytes]. DGU VPO GRMU, 2016; 16.

Torshina V. I. Praktikum po normal'noy fiziologii [Workshop on Normal Physiology]. М.: Publishing House of the Russian University of Friendship peoples, 2014; 56 – 76.

Menshikov V.V. Laboratornyye metody issledovaniya v klinike [Laboratory research methods in the clinic]. – М.: Медицина, 2017; 365.

Romashchenko O.V., V.F. Kamenev. Vozmozhnosti prognozirovaniya sostoyaniya bol'nykh s serdechnoy nedostatochnost'yu po dannym elektronnoy mikroskopii obraztsov krovi. Mir meditsiny i biologii. [Possibilities of predicting the condition of patients with heart failure according to the data of electron microscopy of blood samples.]. World of medicine and biology. 2019 №4. С. 89 – 94. .(in russian) https://doi.org/10.30970/vlubs.2019.81.15. (date of application 14.09.2021) [in Russian]

Berezniakova A. I., Zhemela O. D. Zdatnist do deformatsii membran erytrotsytiv u shchuriv riznoho viku pry hipoksii // Fiziol. zhurnal. 2013; 59(3): 72-77.https://doi.org/10.15407/fz59.03.072. [in Ukranian]

Yanvareva I.N., Pavlova L.P., Baranova T.I., Baksakova G.N.Sistemno-dinamicheskiy podkhod k issledovaniyu adaptatsionnogo potentsiala cheloveka [System-dynamic approach to the study of human adaptive potential].SPb: SPbGU, 2011;105 -154.[in Russian]

Yunkerov V.I., Grigoriev S.G.. Matematiko-statisticheskaya obrabotka dannykh meditsinskikh issledovaniy [Mathematical and statistical processing of medical research data] – SPb.: MMеdА, 2005; 292 [in Russian]

Matthews K., Duffy S. P., Myrand-Lapierre M. E. et al. Microfluidic analysis of red blood cell deformability as a means to assess hemin-induced oxidative stress resulting from Plasmodium falciparum intraerythrocytic parasitism // Integr. Biol. (Camb). 2017. Vol. 9. N 6. P. 519-528.https://doi.org/10.1039/c7ib00039a(date of application 14.09.2021)

Rudakova – Shilina N.K., Matjukova L.D. Ocenka antioksidantnoj sistemy organizma [Assessment of the antioxidant system of the body]. Laboratornoe delo. – 1982. – № 1. – S. 19-22. https://doi.org/10.30970/vlubs.2019.80.04. (In Russian)

Koba L. V., Shapkina O. O., Zhuikova A. Ye., Bondarenko V. A. Stiikist erytrotsytiv shchuriv riznoho viku do riznykh umov seredovyshcha [Resistance of erythrocytes of rats of different ages to different environmental conditions] // Visn. problem biolohii i medytsyny. 2018. Vyp. 3 (145). S. 389-392. https://doi.org/10.29254/2077-4214-2018-3-145-389-392. (date of application 11..06.2021) [in Ukranian].

Alexandre B.M., Charro N., Blonder J. Profiling the erythrocyte membrane proteome isolated from patients diagnosed with chronic obstructive pulmonary disease. J. Proteomics. 2012; 76(2): 259–269. https://doi.org/10.1016/s0378-4274(98)80221-x (date of application 11..06.2021)

Toropchin V.I. Vplyv kombinatsiyi enerlivu ta nukleksa na pokaznyky perekysnoho okyslennya lipidiv u khvorykh na nealkoholʹnyy steatohepatyt, spoluchenyy z syndromom khronichnoyi vtomy [The effect of the combination of energy and nuclex on lipid peroxidation in patients with non-alcoholic steatohepatitis associated with chronic fatigue syndrome]. Ukrainian morphological almanach. 2011;1(9):124-128.[in Ukranian]

Published
2022-06-21
How to Cite
Popel, S., МеlnicI., ChurpiyІ., Bejga, P., & Śliwiński, Z. (2022). Condition of membranes of erythrocytes of peripheral blood of elderly people with chronic tiredness and low level of tolerance to physical load. Health, Sport, Rehabilitation, 8(2), 78-89. https://doi.org/10.34142/HSR.2022.08.02.07
Section
Original articles. Physical therapy